Pathology and Autopsy of a Mouse - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/mouseTissue.html Pathology and Autopsy of a Mouse. Tissue embedding in paraffin, Storage of smears, and slides Formalin fixation. - [Read more]

Decalcification of Animal Tissue Protocol - http://molecool.wustl.edu/ornitzlab/PDFs/Protocols/Decalcification.pdf Decalcification of Animal Tissue Protocol - [Read more]

Collection of Peritoneal Cells - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/CollectPerC.html Collection of Peritoneal Cells Protocol. This protocol has been orginated by Alan M Stall in the Leon and Lenore Herzenberg's Lab at Stanford . Shaking the mouse was found to be necessary for optimum yield of B1 cells. - [Read more]

Protocols for the preparation of tumour cells for subcutaneous injections in mice - http://www.pharma.ethz.ch/institute_groups/biomacromolecules/protocols/tumorcell Protocols for the preparation of tumour cells for subcutaneous injections in mice. - [Read more]

Transplantation of embryonic mammary glands to rescue mammary epithelium from embryonic lethal mutan - http://mammary.nih.gov/tools/mousework/embryo_transp/index.html Transplantation of embryonic mammary glands to rescue mammary epithelium from embryonic lethal mutants. A method for collection and transplantation of mammary anlagen from mouse embryos that survive past embryonic day 13. - [Read more]

Renal Capsule Grafting - http://mammary.nih.gov/tools/mousework/Cunha001/index.html Renal capsule grafting is a method of growing embryonic or neonatal organ rudiments in vivo for extended periods, for maintaining adult tissues in vivo, or for growing neoplastic cells. - [Read more]

Analysis of murine Bone Marrow: specimen choice, sampling and processing. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/AnalysisMurineBM.html Histologic analysis of murine bone marrow is a necessary complement to flow cytometric or in vitro analysis. - [Read more]

CAM preparation Protocol - http://www.pharma.ethz.ch/institute_groups/biomacromolecules/protocols/cam CAM preparation Protocol - [Read more]

Restraint and Handling of Animals General Principles - http://www.ahc.umn.edu/rar/handling.html Information link on proper restraint and handling techniques for animals, in order to reduce stress to animals, but also researchers. - [Read more]

Dissociation of spleen and hemopoietic tissue. - http://icg.cpmc.columbia.edu/cattoretti/Protocol/MousePathology/DissociationSpleen.html Dissociation of spleen and hemopoietic tissue protocol. You need to buy glass slides with frosted, sandblasted ends (Fisher Scientific, Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not be used. Pre-clean the slides by rubbing the edges of the slides against each other in medium, to get rid of the glass powder. Slides can be autoclaved in advance for sterile dissociation. - [Read more]

Isolation of T4 cells from xenograft tumors - http://www.lbl.gov/lifesciences/BissellLab/labprotocols/isolationofcellsfromtumorxenografts.htm Isolation of T4 cells from xenograft tumors - [Read more]