Basic Microscopy
Basic Microscopy
Setting the ocular tubes is essential in order to best fit an individuals own inter-pupillary distance. You should focus on a section at the 10X power using only the right side ocular, by closing your left eye. Then you should repeat this with your opposite eye, using the left ocular, using the focussing device on the barrel of the left side ocular tube.This is done to suit the microscope for your own eyesight.
Setting the irish diaphragm is done in order to obtain the optimum illumination for your miscroscope. This is done be looking approximately 12 inches from the microscope into the barrel and closing the iris diaphragm of the condenser until the circle of light is cut down by one-third, leaving two-thirds of the illumination onto the objective lens.
Oil can be used to improve visibility when using the high power objective.