Immunohistochemistry False Negative Results

Immunohistochemistry False Negative Results can occur when:

1. Wrong antibody is used

2. Antibody is denatured

3. Wrong antibody concentration

4. Antigen is lost through autolysis and/or diffusion (most antigens leak out after fixation, therefore paraffin blocks should be used)

5. Low antigen density that is undetectable by current reagent use and technique