Mancini Test

Mancini Test Protocol

The Mancini Test, also known as the radial immunodiffusion test, is performed using agar with added anti-BSA, to set of predetermined BSA solution concentrations and also to determin the concentration of an unknown sample. The precipitin reaction discs , are measured diameters. These occur, after convering the dish and incubating for a 24 hours period. Using standard curves, the dimater of the precipitating rings for each BSA concentration used can be extrapolated.